Using transrectal ultrasound-guided massage of the accessory sex glands for buck semen collection yields semen with greater cryoresistance than electroejaculation alone during the breeding season

The cryoresistance of bucks’ semen collected by transrectal ultrasound-guided massage the accessory sex glands (TUMASG) and electroejaculation (EE) was compared during breeding season (Study 1) or late non-breeding 2). Semen from 10 Gabon bucks with both methods in each then frozen according to a standardized protocol. time required for ejaculation, number electrical pulses applied, sum applied∗voltage total vocalizations emitted collection animal were recorded. In Study 1, TUMASG longer (P = 0.0006) but fewer lower pulses∗voltage than EE < 0.0001 comparisons). Bucks vocalized times 0001). had greater sperm concentration, mass motility, motile progressive tended have functional membrane 0.02; P 0.003; 0.06, respectively). After freezing-thawing process, quality percentage membrane, normal morphology 0.04; 0.03; 1 almost all variables considered when it EE. 2, 0001; 0.0002, There no differences any variable fresh thawed samples However, other seminal characteristics evaluated did not differ method, there variable. Overall, affected welfare less suitable collecting good high buck. • is collect high-quality season. differ. aid cryoresistance.